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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of this gene has long been observed in several tumors, which ends up in chromosome instability, and thus may perhaps contribute to tumorigenesis. This protein was discovered to affiliate with, and become linked to, the phosphorylation of NPAT protein (nuclear protein mapped towards the ATM locus), which participates in mobile-cycle controlled histone gene expression and plays a important function in endorsing cell-cycle development in the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinct isoforms, have already been explained. Two extra splice variants were reported but in depth nucleotide sequence info is not nevertheless out there.
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Blocking peptides are peptides that bind exclusively towards the focus on antibody and block antibody binding. These peptide usually consists of the epitope regarded because of the antibody.
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This guide outlines more attainable optimization of cellular and immunoassay parameters to make sure the very best final results are...
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This information outlines further more achievable optimization of mobile and immunoassay parameters to be sure the very best final results are...
Antibodies sure to the blocking peptide no longer bind into the epitope over the concentrate on protein. By comparing the staining with the blocked antibody compared to the antibody alone, you can see which staining is specific.
Concentrate on Information and facts Cyclin E belongs on the hugely conserved cyclin household, whose associates are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute into the temporal coordination of every mitotic occasion. Cyclin E sorts a fancy with and functions to be a regulatory subunit of CDK2, whose action is needed for mobile cycle G1/S transition. This protein accumulates on the G1-S stage boundary and is degraded as cells development via S section.
The measurement of protein phosphorylation is a useful gizmo for measuring the modulation of receptor activation by the two antibodies...
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